Envirologix: Putting Science to the Test

QuickStix Test Kit Validations and Testimonials - AS 107

A Monoclonal-Based Lateral Flow Device (LFD) for the Early and Reliable Field Detection of Phakopsora pachyrhizi.

Originally Presented at The American Phytopathological Society’s 2006 National Soybean Rust Symposium, St. Louis, MO (APS), and The 2007 National Plant Diagnostic Network’s National Meeting, Orlando, FL (NPDN) ~ UPDATED at the APS July 2007 Annual Meeting, San Diego, California

The original objective was to determine the sensitivity and specificity of the QuickStix LFD test, performed at Syngenta facilities in Vero Beach, Florida. The updated study sought to demonstrate significant improvement in positive signal intensities across the detection range.

Unique sets of leaves were inoculated with a high and low level of spores, washed, and incubated. Leaves with different symptoms were tested for rust infection with the LFD.

The conclusions proved the LFD is capable of detecting soybean rust before the formation of pustules, with no cross reactivity among diseases most commonly confused with soybean rust, and with significantly higher signal intensities than the previous polyclonal-based format. (click here for full text)



Development of a New Method for the Early Detection of Soybean Rust

Agricultural Technology Innovations S. A. (INTEA)
National Institute of Agricultural Technology (INTA) — Argentina

The objective was to validate the performance of the QuickStix (LFD), and to study the economic benefits of using the LFD to detect and manage soybean rust in Argentine production areas.

Based on conservative assumptions, the study indicates that by minimizing yield loss through timely fungicide application signaled by the LFD, the financial benefit of using the test greatly outweighs the cost. Use of the LFD may help avoid unnecessary preventive chemical treatments, thus saving the producer those costs. It has been proven that the strips can qualitatively identify the disease at an early stage in the field in a quick, simple and practical manner. The strips can detect rust even when chlorotic lesions are very difficult to distinguish under a magnifying glass and is an additional complementary diagnostic tool.

Click here for the complete report in Spanish. Note the file is large and may take time to download.

Click here for the report summary in English.



Testing Kudzu for Soybean Rust
Performed by Dr. Philip Harmon, Univ. of Florida

The QualiPlate™ ELISA kit was positive 6 out of 6 times for infected kudzu leaves, and confirmed on PCR. The healthy kudzu leaves were negative 6 of 6 times. The QualiPlate Kit uses the same antibodies as those in the QuickStix (LFD) kit.

Click here for more validations and testimonials for the QualiPlate Kit for Soybean Rust.


Detailed reports available from EnviroLogix. Call 866-408-4597 (or click here for our online form) to receive a copy.

PDF files, where provided, require Adobe Acrobat Reader.

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