EnviroLogix developed DNAble’s rapid and easy-to-use DNA-based assays using a patented isothermal amplification technology, the first commercialized on-site rapid DNA detection method, and available for sale right now.
DNAble is a very rapid, highly specific isothermal nucleic acid amplification technology that can amplify DNA targets with single base resolution a billion-fold in 5-10 minutes at a constant temperature. A nicking enzyme and a strand-displacing polymerase are used to generate small pieces of DNA that feed a DNA extension reaction; it consists of alternating cycles of nicking and extension processes leading to exponential amplification. This is much faster than the time-consuming thermal cycling that relies on costly equipment.
DNAble offers sensitivity and specificity that is equal to or better than current PCR methods. However, unlike PCR, DNAble shows little inhibitory effects from a crude extraction or exogenous DNA. As a result, DNAble allows for highly sensitive detection with minimal to no sample preparation. Our data confirms the high specificity of the assay with no crossreactivity to closely related bacteria or synthetic mismatch targets.
EnviroLogix has pioneered this technology with your needs in mind. There are three basic formats for detection:
Visual read using an enclosed lateral flow device (LFD)