DNAble^® Molecular Detection

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EnviroLogix developed DNAble’s rapid and easy-to-use DNA-based assays using a patented isothermal amplification technology, the first commercialized on-site rapid DNA detection method, and available for sale right now.

DNAble is a very rapid, highly specific isothermal nucleic acid amplification technology that can amplify DNA targets with single base resolution a billion-fold in 5-10 minutes at a constant temperature. A nicking enzyme and a strand-displacing polymerase are used to generate small pieces of DNA that feed a DNA extension reaction; it consists of alternating cycles of nicking and extension processes leading to exponential amplification. This is much faster than the time-consuming thermal cycling that relies on costly equipment.

DNAble offers sensitivity and specificity that is equal to or better than current PCR methods. However, unlike PCR, DNAble shows little inhibitory effects from a crude extraction or exogenous DNA. As a result, DNAble allows for highly sensitive detection with minimal to no sample preparation. Our data confirms the high specificity of the assay with no cross­reactivity to closely related bacteria or synthetic mismatch targets.

EnviroLogix has pioneered this technology with your needs in mind. There are three basic formats for detection: Visual read using an enclosed lateral flow device (LFD) Read a strip of vials via fluorescence High throughput for trait discovery and seed production

Our first commercially available isothermal molecular detection test (for Cmm), along with multiple contract test kits, showcase DNAble’s many benefits: On-site testing, and portable Minimal sample prep allows for quick preparation; minimal interference from “dirty” samples Low equipment costs, and technicians can easily be trained to run the simple protocol Time to results (TTR) is less than 20 minutes Find out how straightforward molecular diagnostics can be!

Call 1-866-408-4597 or email the DNAble Team for more information on this and our other DNAble-based tests.

New! - EnviroLogix is currently developing multiple DNAble kits in the Veterinary Pathogens area, including E. coli and Salmonella. Click here for more information.