This adventitious presence test kit is intended for seed quality determination by corn breeders and corn seed producers.
Detects: event MON87427.
Sample: crude extract of bulk corn seed, no DNA purification needed.
LOD (Limit of Detection): 1 positive seed in a pool of 200.
Results In: 15 minute assay.
Format: 8 well strips read on the AmpliFire™ portable reader.
Target Trade Names (principal brands): Roundup® Hybridization System (RHS).
Analyte Class: RHS selectively expresses CP4 EPSPS so that male reproductive organs do not express CP4 EPSPS. The only way to distinguish RHS positive seed from Roundup Ready® positive seed is through a molecular test.
- 2x RB1 Reaction Buffer Provided in green 8-well strip tubes (6 strips).
- RHS (MON87427) Master Mix Lyophilized reagents provided in clear 8-well strip tubes (6 strips).
- Flat Caps used for capping the clear tubes prior to assay start (6 strips).
Also required to run test (but not included):
- Pipette tips
- AmpliFire™ 8-well reader
- DNAble® Extraction Set 10
Catalog Number: DF-312
Part Number: 12513