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DNAble Molecular Detection of Roundup Hybridization System (MON87427) in Bulk Corn Seed

Description

This adventitious presence test kit is intended for seed quality determination by corn breeders and corn seed producers.

Detects: event MON87427.

Sample: crude extract of bulk corn seed, no DNA purification needed.

LOD (Limit of Detection): 1 positive seed in a pool of 200.

Results In: 15 minute assay.

Format: 8 well strips read on the AmpliFire™ portable reader.

Target Trade Names (principal brands): Roundup® Hybridization System (RHS).

Analyte Class: RHS selectively expresses CP4 EPSPS so that male reproductive organs do not express CP4 EPSPS. The only way to distinguish RHS positive seed from Roundup Ready® positive seed is through a molecular test.

Kit Components:

  • 2x RB1 Reaction Buffer Provided in green 8-well strip tubes (6 strips).
  • RHS (MON87427) Master Mix Lyophilized reagents provided in clear 8-well strip tubes (6 strips).
  • Flat Caps used for capping the clear tubes prior to assay start (6 strips).

Also required to run test (but not included):

  • Pipettes
  • Pipette tips
  • AmpliFire™ 8-well reader
  • DNAble® Extraction Set 10

 

Catalog Number: DF-312

Part Number: 12513

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