Non-GMO Corn Parental Line Maintenance

Non GMO Testing Made Fast and Easy

Non-GMO Corn Parental Lines

EnviroLogix has easy to use diagnostic tools that identify GMO contamination in parental line seed stocks and in individual plants that are used in conjunction to eliminate GMO contamination in parental line seed stocks. By using EnviroLogix diagnostic tools, GMO contamination is detected and eliminated early in the seed production process thereby increasing the likelihood of producing Non GMO Project compliant commercial seed and grain. Since the EnviroLogix QuickScan platform is commonly used to screen grain in Non GMO food chains, you can trust EnviroLogix tools to identify GMO contamination early in the seed production process and help growers ensure Non GMO Project compliance.

How to use EnviroLogix Kits to Improve Non GMO Corn Parental Line Seed Purity:

  1. Collect a representative seed sample for each corn parental line seed stock.
  2. Screen each parental line seed stock with QuickComb for corn and take note of the contaminating traits specific to each parental line. The visual QuickComb or quantitative QuickComb for Quickscan may be used.
  3. For each parental line, acquire EnviroLogix QuickStix for corn leaf testing that are specific for the contaminating traits that were identified using the QuickComb. See figure below for a table listing the QuickStix and the specific GMO trait detected.
  4. Plant each parental line in an isolated field or greenhouse. Test each plant using the specific QuickStix that were identified for each specific parental line and rogue (remove and destroy) positive plants.
  5. Allow the parental line to self and/or sib and collect seed. Perform typical processing activities (husking, drying, etc.) needed to preserve corn seed for future planting.
  6. Screen the processed seed for GMO contamination using a QuickComb. If pure, proceed to seed multiplication and/or hybrid seed production as needed.

Steps

example image of a QuickComb for QuickScan
  1. Screen each parental line seed stock using the QuickComb. Identify contaminating GMOs (if any) in each parental line seed stock. For example, Cry34 and Vip3A were detected in the QuickComb below.
  2. Order specific strips for detecting the traits that were identified in step (A). The strips will detect the trait of interest in corn leaf. Be sure to order enough strips to test each plant in step (C).
  3. Catalog
    Number
    GMO
    Trait
    Abbreviation
    in Comb
    AS-003-CRLS Cry1Ab C1
    AS-010-LS Roundup Ready RR
    AS-015-LS Cry3Bb C3
    AS-016-LS Cry1F 1F
    AS-014-LS Liberty Link LP
    AS-054-LS Cry34 34
    AS-037-LT mCry3A 3A
    AS-005-LS Cry2A C2
    AS-085-LS Vip3A VP
  4. In an isolated field or greenhouse, plant an appropriate number of seeds for seed multiplication. Prior to pollination, sample true leaf according to the QuickStix product insert instructions and test each plant for the GMO(s) that were detected in step (A). Any plants that are GMO positive should be removed and destroyed. Allow the remaining plants to self and/or sib. Collect and process seed as usual and then confirm Non GMO purity by testing representative sample with QuickComb.