Our simple tests for detecting mycotoxin and GMO proteins are:
- Easy to use
Immunoassay platforms allow for rapid, sensitive and economical screening of large numbers of samples.
The reagents and antibodies used in EnviroLogix test kits are produced on-site in our labs under rigorous quality standards, allowing us to develop and test new diagnostics quickly and efficiently.
How Does It Work?
Immunoassays are based on the scientific principle that specific antigens will stimulate unique immune responses. The proteins produced by the immune response, called antibodies, can be used to signal the presence or absence of a target compound (like a toxin or a GMO) in a sample.
Immunoassay technology can be applied to ELISA plates or tubes, or lateral flow device (LFDs, also known as dipsticks). There are two basic types: sandwich assays, and competitive assays.
In sandwich immunoassays, sample concentration is proportional to color development:
Lighter color = Lower concentration of the target
Darker color = Higher concentration of the target
In competitive assays, the opposite is true: darker color means lower concentration of the target. For more information, read How Immunoassays Work.
Lateral Flow Devices
LFDs employ the same immunoassay principles as the plate and tube formats, but the antibodies and other reagents are coated on a nitrocellulose membrane rather than on the inside of test wells or tubes. The sample travels by capillary action from one end to the other. In passing along the membrane, the sample is exposed to zones of antibody that react to the target analyte. EnviroLogix LFD assays develop a "control line" near the far end of the device to ensure that the test was performed correctly.
As with the plate and tube formats, some tests are competitive assays (such as for mycotoxins) and others are sandwich assays (such as GMOs). In competitive LFD assays, a positive result is indicated by the absence of a line in the test result zone. In sandwich assays, a positive result is indicated by the presence of a line in the test result zone.
LFD tests can be interpreted visually for qualitative results or read using the QuickScan System for traceable, quantitative results.
QuickScan is a highly flexible and precise LFD strip-reading system. Using a touchscreen PC platform, the QuickScan System can process strips for both GMOs and mycotoxins simultaneously. The reader can scan individual strips and multi-strip combs and provides objective, quantifiable results.
By combining digital imaging technology with advanced mathematical processing, test data is analyzed in seconds. The on-board, proprietary software can read, record and store detailed screening results for complete traceability.
- Fast – Read rapid test strips and record results in seconds
- Flexible – Process single strips or multi-strip combs for GMOs or mycotoxins
- Precise – Quantitative standards are integrated into a unique barcode system, ensuring accurate, consistent measurement without calibration
- Traceable – Access test results instantly for emailing, printing, archiving or analyzing trends
EnviroLogix ELISA kits have specific antibodies and different antibodies coated on the sides and bottom of each well (or tube) along with an enzyme linked to a target-specific antibody which produces an antibody-antigen complex in the presence of the target.
Our competitive assays are supplied with Calibrators (concentrations of the target analyte in solution) and a negative Control (free of the target analyte). These Standards exhibit distinctly different shades of color. By using a microtiter plate reader, comparison of the sample produced color against the color produced by the Standards, the person testing may determine the concentration range of the sample in a semi-quantitative or quantitative interpretation.
Sandwich-type ELISA kits are generally supplied with a positive control. The assay is performed including a buffer only in one of the wells to represent what a negative result would look like. To interpret the results of a test, the plate is inserted into a microwell plate reader set to blank on the buffer well. Optical density is then compared between positive control, sample wells, and buffer blank to determine the presence or absence of the target. If known-positive samples are also included, quantitative interpretations may be deduced.