What is the best method for adding the samples to the RB1 tubes?

What is the best method for adding the samples to the RB1 tubes?

When adding the 5 uL of sample to the RB1, add the sample directly into the RB1. Once samples are added to all RB1 tubes, recap tubes with green caps and tap/flick to mix. Remove RB1 caps JUST before transferring to Master Mix tubes.

Set the RB1 tubes in the front of a rack and the Master Mix tubes behind the RB1 tubes.