- Before opening the tube containing the dried down control, lift the tube up to the light to make sure the pellet is at the bottom of the tube. You should see a triangular-shaped drop of material at the bottom of the tube. If the dried down material is missing or has detached from the bottom of the tube, call Technical Support: Toll-free US 1-866-408-4597 ext.2 or (207) 797-0300 ext.2
- Be sure to use the DNAse-free water to re-suspend the control. Add exactly 65 µL of the DNAse-free water with a calibrated pipette.
- It is recommended that the following pipette tips (or equivalent) be used for re-suspending the positive control:
USA Scientific Catalog # 1120-8810. TipOne 1-200 ul aerosol barrier filter pipet tips, sterile, 10 racks of 96 tips (960 tips)
– Filter barrier protects pipettors and samples from contamination
– 50, 100, and 200 µl graduations
– Hydrophobic filters contain no additives and will not absorb samples
– Certified free of detectable RNAse, DNAse, DNA, and pyrogens
– Yellow color-coded tip wafers
- Briefly vortex the re-suspended control to obtain a uniform suspension. Excessive vortexing may compromise the integrity of the control.
- Record the date the control was re-suspended on the tube. Make sure the cap is secured tightly before placing the control back in the refrigerator.
- Do not store the re-suspended control in its original pouch, as the pouch contains a packet of desiccant that may compromise the performance of the control.
- Before using a re-suspended control that has been refrigerated, allow the control to warm to room temperature. Make sure all the liquid is at the bottom of the tube by tapping/flicking the tube. Briefly vortex the control to mix.